The Thermo Scientific Pierce Albumin / IgG Removal Kit is optimized to reduce the abundant albumin and antibody components of the human serum sample in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
Features of the Albumin/IgG Removal Kit:
- Optimized for 2D sample preparation – the microcentrifuge spinning cup method is scaled up and optimized to treat 10 µL human serum samples for 2D electrophoresis
- Efficient: the kit removes almost all HSA and IgG, allowing low abundance proteins to be detected on 2D gels
- Fast: the kit processes samples in less than 40 minutes.
- Scalable – use different amounts of affinity resin to match sample volume and concentration
This dye/protein A kit uses a mixture of Cibacron blue dye and Protein A agarose affinity resin. The dye binds albumin with relatively good specificity and Protein A binds to many species and subclasses of IgG. The Thermo Scientific Pierce Albumin / IgG Removal Kit is inexpensive and can be used to treat specimens from species other than humans (eg, Monkeys, Pigs, Rabbits). The kit is also scalable and sufficient to process 25 samples, each containing 600 µg of total serum protein (approximately 10 µL of serum).
Low abundant proteins in human serum and plasma can provide information on human disease. However, the analysis of human fluids is often complicated by the presence of high concentrations of albumin and IgG, which can constitute more than 70% of the total serum protein (Figure, panel A).
The Thermo Scientific Pierce Albumin / IgG Removal Kit uses a classic blend of Cibacron blue dye and Protein A resin for economical removal of albumin and IgG. This kit can process up to 25 samples of approximately 600 µg of serum using an optimized protocol to minimize nonspecific protein interactions and sample dilution (Figure, panel C).